A simple and sensitive spectrofluorimetric method has been developed for estimation of Febuxostat (FEBU) in tablet dosage form. The thiazole moiety in FEBU acts as fluorophore. FEBU produces strong fluorescence at 378 nm (λem) with excitation at 316 nm (λex) in methanol. Linearity range was found to be 200-1000 ng/ml with mean recovery 100.038%. The correlation co-efficient was found to be 0.9993. The limit of detection (LOD) and limit of quantification (LOQ) for the developed method were found to be 13.406 and 40.625 ng/ml, respectively. The content of FEBU in marketed formulations was found to be 99.37% of labeled amount. The developed method was found to be specific, accurate and precise. It was successfully used for estimation of FEBU in its tablet dosage form.
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